SBEAD monoclonal antibody development platform

SBEAD is a cross species (rabbit, mouse, alpaca and more) platform for monoclonal antibody development. When used in rabbits, our special rabbit strains or transgenic lines can be used to further enhance the antibody capability. The advantages of the platform include:

  • High and broad affinity. The KD values of the most SBEAD developed rabbit mAbs are at or lower than 10^-10M. Low or medium affinity antibodies are also available.
  • Unlimited supply. Customer owns the IP of the selected antibody sequences. Recombinant antibodies can be produced unlimitedly.
  • Fast turnaround. 11 weeks from immunization to the delivery of ELISA positive antibodies for customer’s in house screening. 3 weeks for the sequencing and production of the selected antibodies. (Comparison: single B cell culture method >18 weeks; surface display method >21 weeks; hybridoma method >25 weeks)
  • More clones. >500 ELISA positive antibodies can be provided at customer’s request.
  • Large quantity. μg each recombinant antibodies will be provided for screening, enough for most assays including functional tests. (Comparison: single B cell culture generate mostly <100 ng).
  • Accuracy. Antibody samples are quantified in concentration and antigen binding before they are provided for customer screening.
  • Convenience. Antibodies can be provided with luciferase fusion to facilitate customer’s in house screening

Platform overview

Special

rabbit lines

  • Regular NZW strain
  • LucAb rabbit: transgenic line producing luciferase fused antibodies
  • HuAb rabbit: special strain with high antibody humanization success
  • HyImmune rabbit : transgenic line with enhanced immune response, antibodies can possibly be developed faster (6 weeks)
  • Customized gene knockout rabbit lines: to allow any epitope targeting

Optimized immunization protocol

  • Accelerated 4-week protocol using HyImmune rabbits
  • Classical 2-month protocol
  • Ensure antibody affinity and diversity
  • Serum titer monitored during the process

Rapid
single B cell mRNA amplification

  • 1,000 antigen specific single B cells sorted per day
  • mRNA extraction and amplification in 2 days
  • Guaranteed monoclonality
  • Transfection ready

Efficient
mammalian cell antibody expression

 

  • First batch (96) antibodies obtained in 1 week
  • Up to 500 antibodies can be produced in 1 month
  • μg ELISA positive antibodies provided for screening
  • Antibody concentration and ELISA binding quantified for customer