Highlights:

Sensitive – Low detection limit ≤ 0.5pg/mL
Simple – No secondary antibody needed, one wash step, and no special instruments needed
Fast – 90 minute assay time with only 10 minutes of hands-on time
Stable – Strong, stable signal lasts for hours
Broad – ≥ 4 log dynamic range

This ELISA Kit utilizes ATGC’s proprietary Biologically labeled Ultra-sensitive (BioUltra) antibody for the detection without the use of any chemically conjugated secondary antibodies. The assay requires only one wash step and can be completed within 90 minutes. An antibody specific for SARS-CoV-2 nucleocapsid protein has been pre-coated onto a microplate. Standards and samples are pipetted into the wells together with the BioUltra detection antibody. Any SARS-CoV-2 nucleocapsid protein present forms a complex with the detection antibody and immobilizes to the wells by the coated antibody. Following incubation unbound samples are removed by washing. Then a substrate reagent is added and the luminescent signal is measured with a plate reader. A standard curve is prepared from seven standard dilutions and the SARS-CoV-2 nucleocapsid protein concentration in the sample is determined.

Specifications

Product Type: Kit
Name: BioUltra SARS-CoV-2 Nucleocapsid ELISA Kit
Format: 96-well plate
Components: Antibody coated microplate (12 x 8-well strips)
500x Detection antibody (30 uL)
Recombinant SARS-CoV-2 nucleocapsid protein standard (300 uL, 16 ng/mL in 1x Sample diluent)
Substrate (5 mL)
2x Sample diluent (10 mL)
10x Wash buffer (30 mL)
Tested Applications: Measurement of SARS-CoV-2 nucleocapsid protein in serum, cell culture supernatant and other biological samples.
Storage: -20C to +4C (see product datasheet)
Shipped: Cold packs